Single-round deoxyribozyme discovery
نویسندگان
چکیده
منابع مشابه
Toward the Development of a Single-Round Infection Assay Based on EGFP Reporting for Anti-HIV-1 Drug Discovery
Background: The rapid increase of HIV-1 strains resistant to current antiretroviral drugs is a challenge for successful AIDS therapy. This necessitates the development of novel drugs, and to this end, availability of screening systems for in vitro drug discovery is a priority. Herein, we report the modification of a previously developed system for increased sensitivity, ease of use, and cost-ef...
متن کاملDeoxyribozyme-based half-adder.
We have constructed a solution-phase array of three deoxyribozyme-based logic gates that behaves as a half-adder. Two deoxyribozymes mimic i(1)ANDNOTi(2) and i(2)ANDNOTi(1) gates that cleave a fluorogenic substrate, reporting through an increase in fluorescence emission at 570 nm. The third deoxyribozyme mimics an i(1)ANDi(2) gate and cleaves the other fluorogenic substrate, reporting through a...
متن کاملtoward the development of a single-round infection assay based on egfp reporting for anti-hiv-1 drug discovery
background: the rapid increase of hiv-1 strains resistant to current antiretroviral drugs is a challenge for successful aids therapy. this necessitates the development of novel drugs, and to this end, availability of screening systems for in vitro drug discovery is a priority. herein, we report the modification of a previously developed system for increased sensitivity, ease of use, and cost-ef...
متن کاملDeoxyribozyme-based logic gates.
We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open ...
متن کاملDeoxyribozyme-catalyzed labeling of RNA.
Site-specific covalent modification of RNA is important for enabling structure–function studies. For example, probes such as fluorescein are commonly used in fluorescence resonant energy transfer (FRET) investigations of RNA folding. Biotin is used for immobilization during single-molecule analysis, to enable RNA–protein cross-linking studies, and as a key element of selection schemes in vitro....
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2021
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkab504